Histological Characterization of NMJs
Unleash unprecedented experimental capabilities with Curi Bio’s validated 3D functional model of the human Neuromuscular Junction (NMJ):
Quantifiable Functional Output: Directly measure tissue contractile force in real-time, label-free, and longitudinally using the Mantarray platform.
Selective Neuronal Activation: Elicit light-induced contractions for specific NMJ function measurement, independent of muscle-only effects, using Curi Bio’s optical stimulation device Lasarray.
Scalable & Reproducible Creation: Direct-from-thaw neurosphere creation and in situ neuromuscular co-culture assembly with >95% tissue formation success, optimized for automation and adoption by unspecialized users
Validated Human Physiology: Easy replication of established in vivo responses to both presynaptic (BoNT/A, BoNT/B) and postsynaptic (AChM, Tubocurarine) toxins.
Skeletal Muscle Tissue (blue) featuring arrays of 42 embedded neurospheres (green)
Clear Response: Dose- and time-dependent functional blockade in response to BoNT/A and BoT/B.
Precise & Reproducible EC50: Less than 2% variance (0.11+- 0.015μg) across different users and multiple iPSC differentiations.
Specificity: Loss of NMJ function does not affect underlying muscle contractility.
The first 3D-tissue specific optical stimulation device.
If you are interested in learning more about how Curi Bio’s NMJ model can accelerate your research, please contact us to arrange a conversation with our team of experts.
